It puts the total amount raised by HouseTrip close to $60 million in just short of three years.
Index and Balderton have also participated in the round, HouseTrip says.
Since the November 2011 capital raise, HouseTrip has more than doubled the number of properties available on the site from 55,000 to 130,000.
So what will the company do with its $40 million?
Co-founder and CEO Arnaud Bertrand says the money will be used to grow the company in new territories (expanding on the existing November 2011 geographic strategy), as well as investment in new products and services.
It currently has properties in 15,000 destinations around the world and claims it will have booked three million room nights by the end of this year.
With the likes of HomeAway a dominant player in North America, HouseTrip says it will focus on the European market where the sector is more fragmented.
HouseTrip says the global vacation rental industry is now a staggering $100 billion marketplace.
“My wife and I created HouseTrip on the back of a painful experience booking a holiday rental. We knew there had to be a better way to discover and book holiday rental accommodation that was both anxiety-free and easy-to-use.”